CST, 24903T, NFI-B (E5L7U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NFI-B. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, NFI-B (E5L7U) Rabbit Monoclonal Antibody (CST #24903) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity NFI-B (E5L7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total NFI-B protein. Based on antigen sequence, the antibody is predicted to detect isoform 1 and isoforms 4-6 of NFI-B. Based on sequence homology, this antibody is not predicted to cross-react with other NFI family members. Species cross-reactivity for IF-IC is human only. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro404 of human NFI-B protein. Background NFI-B belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). Research studies have demonstrated that NFI-B plays a crucial role in different aspects of tumor development, acting as an oncogene or tumor suppressor in different types of tumors and participating in tumor-associated fusion gene formation (4-7). Moreover, NFI-B has been shown to promote epithelial-mesenchymal transition and metastatic ability of cancer cells through a number of mechanisms, including upregulating SNAI1 expression in colorectal cancer (8), stabilizing chromatin accessibility in small cell lung cancer (9), and modulating the ZEB1 axis in melanoma (10). Alternate Names CCAAT-box-binding transcription factor; CTF; HMGIC/NFIB; MACID; NF-I/B; NF1-B; NFI-B; NFI-RED; NFIB; NFIB2; NFIB3; Nuclear factor 1 B-type; Nuclear factor 1/B; nuclear factor I B; Nuclear factor I/B; TGGCA-binding protein Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50-75 Source/Isotype: Rabbit IgG