CST, 24923SF, GM130 (D6B1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GM130. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, GM130 (D6B1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #24923) is ready to ship. Product Usage Information This product is the carrier free version of product #12480. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12480 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GM130 (D6B1) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total GM130 protein. This antibody may cross-react with a protein of unknown origin at 30 kDa. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr185 of human GM130 protein. Background The Golgi apparatus functions in the modification, organization, and transport of proteins and membranes targeted to other parts of the cell, such as the plasma membrane, lysosomes, and endosomes. This regulated transport is important for appropriate protein localization, secretion, and signal transduction. Members of the Golgin family of proteins, including GM130, Giantin, p115, and GRASP65, are defined by their presence in the Golgi matrix and by their long coiled-coil domains. Golgin function, which is regulated in part by small GTPases of the Rab and Arl families, includes establishing and maintaining Golgi structure and transport (reviewed in 1). The Golgi cisternae are stacked and linked laterally to form a ribbon. GRASP65 and GM130 are required for membrane fusion events that mediate ribbon formation during Golgi assembly. These lateral fusion events allow for uniform distribution of Golgi enzymes (2). GM130 and Giantin interact with the transport factor p115 to facilitate endoplasmic reticulum (ER)-Golgi transport (3). GM130 is also involved in the transport of the ether-a-go-go-related (hERG) potassium ion channel. Inappropriate hERG localization may be an underlying cause of long QT syndrome, a hereditary and potentially fatal cardiac arrhythmia (4). Further, GM130 was implicated in signal transduction regulating invasion, migration, and cell polarization via its interaction with and activation of serine/threonine kinases YSK1 and Mst4 (5). Alternate Names 130 kDa cis-Golgi matrix protein; GM130; GM130 autoantigen; GOGA2; GOLGA2; golgi autoantigen, golgin subfamily a, 2; Golgi matrix protein GM130; golgin A2; Golgin subfamily A member 2; Golgin-95; MGC20672; SY11 protein Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG