CST, 2494S, MALT1 Antibody

Polyclonal Antibody for studying MALT1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, MALT1 Antibody (CST #2494) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MALT1 Antibody detects endogenous levels of total MALT1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of mouse and rat MALT1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Mucosa-associated lymphoid tissue translocation gene 1 ( ) is a paracaspase that is a critical mediator of T-cell receptor activation of NF-κB and may contribute to the progression of MALT lymphomas (1-4). It contains two immunoglobulin-like domains, an amino-terminal death domain and a carboxy-terminal caspase-like domain. Association of MALT1 with Bcl-10 and CARD11/Carma1 leads to activation of IKK and subsequent stimulation of NF-κB, resulting in increased proliferation and inhibition of apoptosis (5,6). A common translocation in MALT B-cell non-Hodgkin lymphomas t(11;18)(q21;q21) results in the fusion of the amino terminus of API2 (c-IAP2), a member of the inhibitor of apoptosis protein family, to the carboxy terminus of MALT1 (1,2). The API2-MALT1 fusion protein likely leads to deregulation of NF-κB, contributing to increased oncogenic potential (7). Alternate Names caspase-like protein; DKFZp434L132; IMD12; MALT associated translocation; MALT lymphoma-associated translocation; MALT-lymphoma associated translocation; MALT1; MALT1 paracaspase; MALT1 protease; MLT; MLT1; mucosa associated lymphoid tissue lymphoma translocation gene 1; Mucosa-associated lymphoid tissue lymphoma translocation protein 1; Paracaspase; paracaspase-1; PCASP1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 SOURCE: Rabbit