CST, 24961S, NF-kappaB1 p105/p50 (D4P4D) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying NFkB-p105 mouse. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, NF-kappaB1 p105/p50 (D4P4D) Rabbit Monoclonal Antibody (PE Conjugate) (CST #24961) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity NF-kappaB1 p105/p50 (D4P4D) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total NF-κB1 p105/p50 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile415 of mouse NF-κB1 p105/p50 protein. Background Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). Alternate Names DNA-binding factor KBF1; EBP-1; NF kappaB1; NF-k; NF-kappa-B1 p84/NF-kappa-B1 p98; NF-kappaB; NF-kappaB p50; NF-kappaB1; NF-KB1; NFkB-p105; Nfkb1; nuclear factor kappaB p50; Nuclear factor NF-kappa-B p105 subunit; Nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light chain gene enhancer in B-cells 1, p105; nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, p105; nuclear factor-kappaB p50; OTTMUSP00000018024; p105; p50; p50 subunit of NF kappaB; p50 subunit of NF-kappaB; p50/p1; p50/p105 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG