CST, 25000S, USB1 (F4R5D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying USB1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, USB1 (F4R5D) Rabbit Monoclonal Antibody (CST #25000) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity USB1 (F4R5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total USB1 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human USB1 protein. Background U6 biogenesis 1 (USB1), also known as MPN1, is a 3'-5' RNA exonuclease that removes uridines and adenosines from the 3' end of U6 snRNA, creating a 2', 3'-cyclic phosphate formation, which protects U6 snRNA from degradation by the nuclear exosome (1-3). USB1 measures the U6 oligo(U) tail by reading the A102 nucleotide position and pauses five uridine residues downstream (3). Loss of USB1 results in a rare condition called poikiloderma with neutropenia, where U6 snRNA is unstable and contains aberrantly long and oligoadenylated 3' ends, resulting in splicing dysfunction (1,4,5). Alternate Names C16orf57; chromosome 16 open reading frame 57; CP057; FLJ13154; hUsb1; HVSL motif containing 1; HVSL1; hypothetical protein LOC79650; Mpn1; mutated in poikiloderma with neutropenia protein 1; PN; putative U6 snRNA phosphodiesterase; U six biogenesis 1; U6 snRNA biogenesis 1; U6 snRNA biogenesis phosphodiesterase 1; U6 snRNA phosphodiesterase; U6 snRNA phosphodiesterase 1; UPF0406 protein C16orf57; USB1 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 25, 28 Source/Isotype: Rabbit IgG