CST, 25009T, Phospho-SF3B1 (Thr313) (D8D8V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SF3B1 (Thr313) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-SF3B1 (Thr313) (D8D8V) Rabbit Monoclonal Antibody (CST #25009) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-SF3B1 (Thr313) (D8D8V) Rabbit Monoclonal Antibody recognizes endogenous levels of SF3B1 protein only when phosphorylated at Thr313. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr313 of human SF3B1 protein. Background Splicing factor 3b subunit 1 (SF3B1) is an integral component of the U2 small nuclear ribonucleoprotein (U2 snRNP) and plays an important role in the splicing of pre-mRNA that involves the removal of introns and the joining of exons to form mature mRNA (1-3). The assembly and proper recognition of splice sites are driven by sequences at the pre-mRNA intron-exon splice sites. The 5' splice donor site is recognized by the U1 snRNP complex, while U2 snRNP binds to the 3' splice site (branch point), ensuring the anchoring of the spliceosome machinery at the splice sites (3,4). Recent whole exome sequencing studies have demonstrated a high incidence of somatic mutations of in patients with various hematological malignancies such as chronic lymphocytic leukemia and myelodysplastic syndromes (2,3,5,6). Misregulation of pre-mRNA splicing arising from mutations of the spliceosome components such as SF3B1 is thought to contribute to changes in the expression patterns of key proteins that are involved in pathways such as cell cycle progression, cell death, and cancer metabolism (2,3). Phosphorylation of SF3B1 at Thr313 is only found in catalytically active spliceosomes and associates mainly with chromatin, where about 80% of pre-mRNA splicing occurs. Treatment with a transcription inhibitor 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole (DRB) leads to a decreased supply of pre-mRNA, resulting in the loss of phospho-SF3B1 (Thr313), consistent with its association with active splicing (7). Alternate Names Hsh155; MDS; pre-mRNA processing 10; pre-mRNA splicing factor SF3b, 155 kDa subunit; Pre-mRNA-splicing factor SF3b 155 kDa subunit; PRP10; PRPF10; SAP 155; SAP155; SF3B1; SF3b155; spliceosome associated protein 155; Spliceosome-associated protein 155; Splicing factor 3B subunit 1; splicing factor 3b, subunit 1, 155kDa Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 155 Source/Isotype: Rabbit IgG