CST, 25084S, Cathepsin S Antibody

Polyclonal Antibody for studying CTSS. Validated for Western Blotting,Simple Western™. Highly specific and rigorously validated in-house, Cathepsin S Antibody (CST #25084) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cathepsin S Antibody recognizes endogenous levels of total cathepsin S protein. This antibody detects the full-length pro-protein (37 kDa), and proteolytically activated cathepsin S (25 kDa). In some cell extracts, the antibody weakly detects a 70 kDa protein of unknown identity. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly233 of human cathepsin S protein. Antibodies are purified by peptide affinity chromatography. Background Cathepsin S (CTSS) is a cysteine protease belonging to the cathepsin family (1). It is synthesized as a 37 kDa pro-protein, which is proteolytically processed to remove the amino terminal activation peptide, yielding the mature (active) cathepsin S protein (2). The protein is localized to the lysosomal or endosomal compartments of antigen-presenting cells such as B cells, macrophages, and dendritic cells, where it plays an important role in immune response activation (3,4). Specifically, cathepsin S proteolytically removes the self-associated invariant chain (CD74) from MHC class II, and promotes MHC-exogenous antigenic peptide loading (5). Cathepsin S may also be secreted by cells to the extracellular matrix, where its proteolytic degradation of matrix proteins and cytokines (e.g., collagens, elastin, PAR2) has been shown to influence cell signaling (1). Aberrant expression and activity of cathepsin S have been associated with various disease states, including atherosclerosis, fibrosis and inflammation, autoimmune disease, and cancer (6-10), making it a potential biomarker and a promising therapeutic target. Alternate Names Cathepsin S; CATS; CTSS; MGC3886 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 25, 37 SOURCE: Rabbit