CST, 2509S, AP-2 beta Antibody

Polyclonal Antibody for studying AP-2beta. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, AP-2 beta Antibody (CST #2509) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity AP-2 beta Antibody detects endogenous levels of total AP-2β protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human AP-2β. Antibodies are purified by protein A and peptide affinity chromatography. Background The sequence-specific transcription factor activator protein 2β (AP-2β) is required for normal kidney development (1). AP-2β knockout mice die 1-2 days after birth due to polycystic kidney disease (1). Massive apoptosis occured during kidney deveopment at the end of embryogenesis in these mice (1). Overexpressed AP-2β has been to found to suppress c-myc-induced apoptosis, indicating a role of this transcription factor in cell survival (1). In addition, overexpression of AP-2β is shown to be related to impaired insulin signaling in adipocytes, and is therefore proposed to be a candidate gene that may relate to obesity (2). Alternate Names activating enhancer binding protein 2 beta; Activating enhancer-binding protein 2-beta; AP-2 beta; AP-2B; AP2-B; AP2-beta; AP2B; MGC21381; PDA2; TFAP2B; transcription factor AP-2 beta; transcription factor AP-2 beta (activating enhancer binding protein 2 beta); Transcription factor AP-2-beta Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 49 SOURCE: Rabbit