CST, 2510S, TrkA (12G8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TrkA. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, TrkA (12G8) Rabbit Monoclonal Antibody (CST #2510) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 86180 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity TrkA (12G8) Rabbit Monoclonal Antibody detects endogenous levels of total TrkA protein. This antibody does not cross-react with TrkB. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Arg220 of human TrkA. Background The family of Trk receptor tyrosine kinases consists of TrkA, TrkB, and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3 (1). Neurotrophin signaling through these receptors regulates a number of physiological processes, such as cell survival, proliferation, neural development, and axon and dendrite growth and patterning (1). In the adult nervous system, the Trk receptors regulate synaptic strength and plasticity. TrkA regulates proliferation and is important for development and maturation of the nervous system (2). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade (3,4). Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at these sites reflects TrkA kinase activity (3-6). Point mutations, deletions, and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA (7-10). TrkA is activated in many malignancies including breast, ovarian, prostate, and thyroid carcinomas (8-13). Research studies suggest that expression of TrkA in neuroblastomas may be a good prognostic marker as TrkA signals growth arrest and differentiation of cells originating from the neural crest (10). Alternate Names DKFZp781I14186; gp140trk; High affinity nerve growth factor receptor; MTC; neurotrophic receptor tyrosine kinase 1; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; Oncogene TRK; p140-TrkA; TRK; Trk-A; TRK1; TRK1-transforming tyrosine kinase protein; TRKA; Tropomyosin-related kinase A; Tyrosine kinase receptor; tyrosine kinase receptor A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG