CST, 25121SF, CD163 (D6U1J) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CD163. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CD163 (D6U1J) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #25121) is ready to ship. Product Usage Information This product is the carrier free version of product #93498. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 93498 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CD163 (D6U1J) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD163 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the human CD163 protein. Background CD163 is a transmembrane scavenger receptor expressed on the macrophage surface. It has 9 B-type Scavenger Receptor Cysteine-Rich (SRCR) extracellular domains mediating serum haptoglobin clearing/endocytosis, pathogen binding and signal transduction, and calcium binding (1,2). CD163 is used as a surface marker of M2 type macrophages, including M2 type tumor-associated macrophages (TAMs), which facilitate cancer progression by secreting cytokines to promote angiogenesis, immunosuppression, and metastasis (3). Inflammatory stimulation and stress signals can induce extracellular domain shedding of CD163 to generate soluble CD163 (sCD163). The increased sCD163 level in serum is associated with low-grade inflammation in disease conditions (4-7). Alternate Names C163A; CD163; CD163 antigen; CD163 molecule; hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; SCARI1; Scavenger receptor cysteine-rich type 1 protein M130; sCD163; soluble CD163 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 160, 170 Source/Isotype: Rabbit IgG