CST, 25162SF, NG2/CSPG4 (E3B3G) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NG2. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, NG2/CSPG4 (E3B3G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #25162) is ready to ship. Product Usage Information This product is the carrier-free version of product #43916. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 43916 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NG2/CSPG4 (E3B3G) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NG2/CSPG4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp1410 of human NG2/CSPG4 protein. Background The chondroitin sulfate proteoglycan NG2 is a type I membrane protein expressed by subpopulations of glia, including oligodendroglial precursor cells and a variety of tumor cells. Normal precursor cells and malignant tumor cells migrate and proliferate, but there is evidence that cells may not be able to engage in both activities at the same time. However, NG2 is involved in promoting both proliferation and motility (1). The extracellular domain of NG2 sequesters growth factors and binds to both growth factor receptors and extracellular matrix ligands such as fibronectin, collagens, and laminin. The cytoplasmic domain is involved in activating Rac, Cdc42, and p130 Cas (2). PKCα phosphorylates NG2 at Thr2256, triggering the redistribution of NG2 from apical microprocesses to lamellipodia, accompanied by enhanced cell motility (3). ERK phosphorylates NG2 at Thr2314, stimulating cell proliferation (4). Alternate Names Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated); Chondroitin sulfate proteoglycan NG2; CSPG4; CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG; Melanoma chondroitin sulfate proteoglycan; Melanoma-associated chondroitin sulfate proteoglycan; MSK16; NG2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 250, 450 Source/Isotype: Rabbit IgG