CST, 25177S, ATF-1 Antibody

Polyclonal Antibody for studying ATF-1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, ATF-1 Antibody (CST #25177) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ATF-1 Antibody recognizes endogenous levels of total ATF-1 protein. This antibody may detect bands of unknown identity at 130-150 kDa. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ATF-1 protein. Antibodies are purified by peptide affinity chromatography. Background Activating transcription factor 1 (ATF-1) is a bZIP transcription factor that is a member of the cyclic AMP (cAMP)-response element-binding protein (CREB) family. Like other members of the CREB family, ATF-1 mediates signals from numerous physiological stimuli and activates target genes through cAMP-response elements, resulting in the regulation of a broad array of cellular responses. ATF-1 can be phosphorylated at Ser63 by protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), and cyclin-dependent kinase 3 (CDK3), which recruits CBP/p300 and enhances transcriptional activity (1-3). ATF-1 expression levels have been found to be elevated in lymphomas and metastatic melanoma cells, where it contributes to tumor growth (4,5). The gene fusion of with , which encodes for the EWSR1-ATF1 oncoprotein, is also implicated in a variety of cancers, most notably in clear cell sarcoma (CCS) (6). Alternate Names Activating transcription factor 1; ATF-1; ATF1; cAMP-dependent transcription factor ATF-1; Cyclic AMP-dependent transcription factor ATF-1; EWS-ATF1; FUS/ATF-1; Protein TREB36; TREB36 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit