CST, 2519S, TGF-beta Receptor III Antibody

Polyclonal Antibody for studying TGFBR3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, TGF-beta Receptor III Antibody (CST #2519) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TGF-beta Receptor III Antibody detects endogenous levels of the type III TGF-β receptor. This antibody does not cross-react with other family members at physiological conditions. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues in the extracellular domain of the type III TGF-β receptor. Antibodies are purified by protein A and peptide affinity chromatography. Background Transforming growth factor-β (TGF-β) proteins belong to the TGF-β superfamily of cytokines that play a critical role in regulating cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-3). TGF-β ligands elicit signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII) TGF-β receptors. Type I and type II receptors are serine/threonine kinases that form a heteromeric complex following ligand binding to the type II receptor. In response to ligand binding, the type II receptors form a stable complex with the type I receptors, triggering phosphorylation and activation of the type I receptor (4). This results in the recruitment of receptor-mediated SMADs (SMAD2, SMAD3), which are phosphorylated by the type I kinase in an SSXS domain in the C-terminus. This leads to recruitment of the co-SMAD (SMAD4), and subsequent translocation of this heteromeric SMAD complex to the nucleus, where it regulates transcription of target genes (5-7). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain. Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex (8). The type III TGF-β receptor is upregulated during skeletal muscle differentiation (10). Alternate Names Betaglycan; betaglycan proteoglycan; BGCAN; TGBR3; TGF-beta receptor type 3; TGF-beta receptor type III; TGFBR3; TGFR-3; transforming growth factor beta receptor 3; Transforming growth factor beta receptor III; Transforming growth factor beta receptor type 3; transforming growth factor, beta receptor III Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 110 SOURCE: Rabbit