CST, 25285SF, DEPTOR/DEPDC6 (D9F5) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Deptor. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, DEPTOR/DEPDC6 (D9F5) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #25285) is ready to ship. Product Usage Information This product is the carrier free version of product #11816. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 11816 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity DEPTOR/DEPDC6 (D9F5) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total DEPTOR/DEPDC6 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu113 of human DEPTOR/DEPDC6 protein. Background DEPTOR/DEPDC6 is a component of both mTORC1 and mTORC2 complexes (1). It interacts with mTOR and inhibits mTORC1 and mTORC2 kinase activities (1). mTOR, along with casein kinase I, phosphorylates DEPTOR/DEPDC6 in the presence of growth signals, which leads to the degradation of DEPTOR/DEPDC6 (2). Research studies have shown that transgenic mice overexpressing DEPTOR/DEPDC6 have more white adipose tissue, and DEPTOR/DEPDC6 expression levels increase in the white adipose tissue of obese humans (3). Furthermore, the expression of DEPTOR/DEPDC6 is induced during mouse adipocyte differentiation (3). Together these findings suggest that DEPTOR/DEPDC6 regulates adipogenesis (3). Alternate Names DEP domain containing 6; DEP domain containing mTOR interacting protein; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; DEP.6; DEPD6; DEPDC6; Deptor; DKFZp564B1778; DPTOR; FLJ12341; FLJ12428; FLJ13854 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG