CST, 25314S, N4BP1 (E8H5E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying N4BP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, N4BP1 (E8H5E) Rabbit Monoclonal Antibody (CST #25314) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity N4BP1 (E8H5E) Rabbit Monoclonal Antibody recognizes endogenous levels of total N4BP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu405 of human N4BP1 protein. Background N4BP1 (NEDD4-binding protein 1) was identified as a binding partner and substrate for the HECT domain-containing ubiquitin ligase NEDD4 (1). Subsequently, it was also found to interact with Itch, a ubiquitin ligase structurally similar to NEDD4 (2). Interaction between N4BP1 interferes with the ability of Itch to bind its substrates, including c-Jun and p63 (2). N4BP1 expression has been associated with immune regulation as well as cancer. N4BP1 was identified as an inhibitory factor of NF-κB-mediated MHC-1 expression in neuroblastoma and high expression of N4BP1 was linked to poor overall survival (3). Studies have also found that N4BP1 is a suppressor of cytokine production that can be cleaved and inactivated by caspase-8 (4). N4BP1 can interact with the NF-κB modulator NEMO to suppress NF-κB transcriptional activity (5). Studies have also identified N4BP1 as a potent interferon-inducible HIV-1 restriction factor that targets the degradation of viral mRNA (6). Alternate Names FLJ31821; KIAA0615; MGC176730; N4BP1; NEDD4 binding protein 1; NEDD4-binding protein 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Rabbit IgG