CST, 25334T, Integrin beta8 (F1H4B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ITGB8. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Integrin beta8 (F1H4B) Rabbit Monoclonal Antibody (CST #25334) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Integrin beta8 (F1H4B) Rabbit Monoclonal Antibody recognizes endogenous levels of total integrin β8 protein. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human integrin β8 protein. Background Integrin αvβ8 facilitates activation and release of TGF-β, which has immunosuppressive effects (1). Deletion of integrin β8 from dendritic cells led to development of inflammatory bowel disease and autoimmunity in mice, as well as failure to induce regulatory T cells that require TGF-β for development (2). TGF-β is also involved in Th17 cell differentiation. Mice lacking expression of integrin β8 on dendritic cells fail to develop Th17 cells and are protected from Th17-mediated autoimmune disease (3). Regulatory T cells express high levels of integrin αvβ8, which enables them to suppress pathogenic T cell activation by activating latent TGF-β (4). Integrin β8 is also essential for vascular morphogenesis as integrin β8-deficient mice are embryonic or perinatal lethal and exhibit insufficient and abnormal vascular morphogenesis (5). Alternate Names Integrin beta-8; integrin subunit beta 8; integrin, beta 8; ITB8; ITGB8 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 98 Source/Isotype: Rabbit IgG