CST, 2534T, GLI1 (V812) Antibody

Polyclonal Antibody for studying GLI1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GLI1 (V812) Antibody (CST #2534) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GLI1 (V812) Antibody detects the endogenous levels of total GLI1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val812 of human GLI1. Antibodies are purified by peptide affinity chromatography. Background GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Krüppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5). Alternate Names GLI; GLI family zinc finger 1; GLI-Kruppel family member GLI1; GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; PAPA8; PPD1; Zinc finger protein GLI1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 160 SOURCE: Rabbit