CST, 25374SF, Lefty1 (D7E3G) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying LEFTY1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Lefty1 (D7E3G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #25374) is ready to ship. Product Usage Information This product is the carrier free version of product #12647. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12647 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Lefty1 (D7E3G) Rabbit Monoclonal Antibody recognizes transfected levels of total lefty1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro108 of human lefty1 protein. Background Lefty proteins are members of the TGF-β family of cell signaling molecules that are involved in growth and development (1,2). Named for their role in left-right axis determination and their exclusive expression on the left side of the developing mouse embryo, lefty1 and lefty2 contain a cysteine-knot motif that is characteristic of TGF-β related proteins, but lack an alpha-helix and a cysteine residue critical for ligand dimerization (3). Early in vertebrate embryogenesis, lefty represses TGF-β signaling by inhibiting the phosphorylation of Smad2 following activation of the TGF-β receptor (4). Down-regulated very early upon differentiation (5), lefty proteins act as extracellular antagonists of the signaling pathway for Nodal, a TGF-β ligand critical for left-right patterning and formation of the mesoderm and endoderm (6). Similar to other members of the TGF-β superfamily, lefty proproteins undergo cleavage to release a bioactive protein (7). The biologically active 42 kDa lefty precursor and the 28 kDa polypeptide have been shown to induce MAPK activity (7). Alternate Names BMP-17; BMP17; Left-right determination factor 1; Left-right determination factor B; LEFTB; lefty b; lefty-1; lefty-b; lefty1; leftyb; LFTY1; Protein lefty-1; Protein lefty-B; stra3 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 40 Source/Isotype: Rabbit IgG