CST, 25401S, HNF-1 alpha (E3H9V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HNF1alpha. Validated for Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HNF-1 alpha (E3H9V) Rabbit Monoclonal Antibody (CST #25401) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity HNF-1 alpha (E3H9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total HNF1α protein. This antibody does not cross-react with HNF1β protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala298 of human HNF1α protein. Background Hepatocyte nuclear factor 1α (HNF1α, also known as TCF1 or MODY3) is a transcription factor that plays a role in the tissue-specific regulation of liver gene expression (1). Research has shown that heterogeneous mutations of HNF1α are linked to maturity onset diabetes of the young (MODY) (2). Recent studies indicate that increased concentrations of free fatty acids can reduce the expression of FoxA2/HNF3β and HNF1α in pancreatic β-cells and lead to their nuclear exclusion, resulting in symptoms of several metabolic syndromes (3). Alternate Names albumin proximal factor; hepatic nuclear factor 1; Hepatocyte nuclear factor 1-alpha; HNF-1-alpha; HNF-1A; HNF1; HNF1 homeobox A; HNF1A; HNF4A; IDDM20; interferon production regulator factor; LF-B1, hepatic nuclear factor (HNF1), albumin proximal factor; LFB1; Liver-specific transcription factor LF-B1; MODY3; TCF-1; TCF1; Transcription factor 1; transcription factor 1, hepatic; truncated hepatocyte nuclear factor 1 alpha Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 81 Source/Isotype: Rabbit IgG