CST, 2541S, Phospho-Paxillin (Tyr118) Antibody

Polyclonal Antibody for studying Paxillin (Tyr118) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Paxillin (Tyr118) Antibody (CST #2541) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Paxillin (Tyr118) Antibody detects endogenous levels of paxillin only when phosphorylated at tyrosine 118. This antibody does not cross-react with other tyrosine phosphorylated proteins. Species Reactivity: Human, Mouse, Rat, Monkey, Pig Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr118 of human paxillin. Antibodies are purified by protein A and peptide affinity chromatography. Background Paxillin is a multidomain protein that localizes primarily to focal adhesion sites in the extracellular matrix (1). Paxillin is one of the key components of integrin signaling, and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by another focal adhesion component, focal adhesion kinase (FAK), at Tyr118 (3,4). Phospho-paxillin (Tyr118) may provide a docking site for recruitment of other signaling molecules to focal adhesions. It has been shown that the SH2 domain of Crk binds to the phosphorylated Tyr118 of paxillin (5). Alternate Names FLJ16691; PAXI; Paxillin; PXN; testicular tissue protein Li 134 Specification REACTIVITY: H M R Mk Pg SENSITIVITY: Endogenous MW (kDa): 68 SOURCE: Rabbit