CST, 25484T, TMEM164 (F2M4W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TMEM164. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TMEM164 (F2M4W) Rabbit Monoclonal Antibody (CST #25484) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TMEM164 (F2M4W) Rabbit Monoclonal Antibody recognizes endogenous levels of total TMEM164 protein. This antibody detects a 120 kDa protein of unknown identity in some cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro76 of human TMEM164 protein. Background Transmembrane protein 164 (TMEM164) is a member of the TMEM family of proteins that span the lipid bilayer of the plasma membrane and other cellular organelles (1). TMEM164 has been shown to act as a positive regulator of ferroptosis by selectively mediating autophagy-related 5 (Atg5)-dependent autophagosome formation during ferroptosis, rather than starvation. Induced by ferroptosis activators, such as RSL3, this TMEM164-dependent autophagosome formation results in the selective degradation of anti-ferroptosis regulators, including GPX4, FTH1, and lipid droplets (2). Additionally, TMEM164 has been identified as an acyltransferase involved in the synthesis of specific ether phospholipids which also contribute to ferroptosis (3). High TMEM164 expression is associated with improved survival and increased immune cell infiltration in patients with pancreatic cancer (2), whereas expression is downregulated in lung cancer and associated with poor prognosis (4). Therefore, upregulation of TMEM164 as a therapeutic target can significantly inhibit cell proliferation, migration, and invasion. Notably, high TMEM164 expression combined with anti-PD-1 antibodies demonstrated synergistic anti-tumor effects in mouse models (4,5). TMEM164 overexpression was also shown to prevent the induction of neurotoxic reactive astrocytes, amyloid β deposition, neurodegeneration, and memory decline in the 5XFAD Alzheimer's disease mouse model (6). Alternate Names bB360B22.3; FLJ20173; FLJ22679; RP13-360B22.2; TM164; TMEM164; Transmembrane protein 164 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG