CST, 2548S, EDC4/Ge-1 Antibody

Polyclonal Antibody for studying GE-1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, EDC4/Ge-1 Antibody (CST #2548) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity EDC4/Ge-1 Antibody detects endogenous levels of total EDC4/Ge-1 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human EDC4/Ge-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Enhancer of mRNA decapping 4 (EDC4) was originally identified as the autoantigen Ge-1 from a Sjögren's syndrome patient later diagnosed with primary biliary cirrhosis (1). EDC4 (also known as HEDLS) was later identified as an essential component of cytoplasmic P-bodies responsible for mRNA decapping and degradation (2). Identified EDC4 protein is found as a pair of isoforms generated by alternative splicing and contains several WD domains and a putative nuclear localization signal. EDC4 co-localizes with other P-body decapping proteins such as DCP1A, DCP2 and GW182 (2,3). Experimental evidence suggests that EDC4 may be involved in miRNA-mediated translation repression (4). Alternate Names Autoantigen Ge-1; Autoantigen RCD-8; EDC4; enhancer of mRNA decapping 4; Enhancer of mRNA-decapping protein 4; Ge-1; GE1; HEDL5; HEDLS; Human enhancer of decapping large subunit; RCD-8; RCD8 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 170 SOURCE: Rabbit