CST, 25575T, Tau 0N (F3K1E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tau iso2 (N0). Validated for WB,WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Tau 0N (F3K1E) Rabbit Monoclonal Antibody (CST #25575) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:500 - 1:2000 Immunofluorescence (Frozen): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Tau 0N (F3K1E) Rabbit Monoclonal Antibody recognizes endogenous levels of total tau isoforms 2 and 6 (tau 0N). This antibody does not cross-react with tau isoforms 4, 5, 7, or 8. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala45 of human tau protein isoform 2. Background Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). Alternative splicing on exons 2 and 3 results in the number of amino (N)-terminal inserts yielding 0N, 1N, or 2N tau isoforms. 0N3R, also called fetal tau or isoform 2, is highly present during brain development in both humans and mice (4). 0N4R, or isoform 6, is abundant in pathogenic insoluble tau fractions of AD, suggesting this isoform may be prone to aggregation in AD (5). Alternate Names DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MAPTL; MGC138549; microtubule associated protein tau; Microtubule-associated protein tau; MSTD; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; TAU; Tau iso2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50-80 Source/Isotype: Rabbit IgG