CST, 2559S, EGR3 Antibody

Polyclonal Antibody for studying EGR3. Validated for Western Blotting. Highly specific and rigorously validated in-house, EGR3 Antibody (CST #2559) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity EGR3 Antibody recognizes endogenous levels of total EGR3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe160 of human EGR3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Early growth response 3 (EGR3) is a zinc finger transcription factor and one of four members of the early growth response family (1). Members of this family are basally expressed in the brain (2) and are immediate early response genes that are important for the induction of cellular programs of differentiation, proliferation, and cell death in response to environmental stimuli (1). EGR3 plays important roles in cellular growth and neuronal development (2) and an essential role in learning and memory processing of both short- and long-term hippocampus-dependent memory; it also mediates adaptation to stress and novelty (3). Research studies show that a lack of EGR3 results in abnormal neuronal and T cell development (4). Alternate Names early growth response 3; Early growth response protein 3; EGR-3; EGR3; MGC138484; PILOT; Zinc finger protein pilot Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit