CST, 2561S, Phospho-CDK2 (Thr160) Antibody

Polyclonal Antibody for studying Cdk2 (Thr160) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-CDK2 (Thr160) Antibody (CST #2561) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-CDK2 (Thr160) Antibody detects endogenous levels of CDK2 only when phosphorylated at threonine 160. This antibody weakly cross-reacts with cdc2 phosphorylated at Thr161. This antibody does not cross-react with other phosphorylated cyclin dependent kinases. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr160 of human CDK2. Antibodies are purified by protein A and peptide affinity chromatography. Background Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5). Alternate Names cdc2-related protein kinase; CDK2; CDKN2; cell devision kinase 2; Cell division protein kinase 2; cyclin dependent kinase 2; Cyclin-dependent kinase 2; p33 protein kinase; p33(CDK2) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 33 SOURCE: Rabbit