CST, 25638T, HPCAL1 (F5R3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HPCAL1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HPCAL1 (F5R3N) Rabbit Monoclonal Antibody (CST #25638) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity HPCAL1 (F5R3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total HPCAL1 protein. HPCAL1 (F5R3N) Rabbit Monoclonal Antibody lacks sensitivity in some low-expressing cell lines by immunofluorescence. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human HPCAL1 protein. Background Hippocalcin-like 1 (HPCAL1), also known as visinin-like protein 3 (VILIP-3), is a neuron-specific calcium-binding protein, primarily found in retina and brain, which is involved in calcium-dependent signal transduction and neuronal differentiation (1). Through its non-canonical calcium-independent role, HPCAL1 also functions as a specific autophagy receptor that drives ferroptosis by facilitating N-cadherin degradation, preserving membrane integrity, and promoting lipid peroxidation (2). HPCAL1 has been implicated in both tumor-suppressing and tumor-promoting roles depending on the cancer type. In hepatocellular carcinoma (HCC), HPCAL1 acts as a tumor suppressor by stabilizing cell cycle regulators, suppressing mTOR signaling, and inhibiting lipid metabolism (3). Loss or downregulation of HPCAL1 has also been observed in HCC patients (4). Conversely, studies have shown that HPCAL1 promotes tumor progression in glioblastoma via activation of the Wnt/β-catenin pathway (5) and in non-small cell lung carcinoma via activation of lactate dehydrogenase A (LDHA) (6). Elevated HPCAL1 expression is also correlated with poor prognosis in both glioblastoma (7) and cholangiocarcinoma (8), further supporting its potential as a novel proto-oncogene. Alternate Names BDR1; Calcium-binding protein BDR-1; hippocalcin like 1; hippocalcin-like 1; Hippocalcin-like protein 1; HLP2; HPCAL1; HPCL1; VILIP-3; Visinin-like protein 3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 20 Source/Isotype: Rabbit IgG