CST, 2564S, RhoGDI Antibody

Polyclonal Antibody for studying RhoGDI alpha. Validated for Western Blotting. Highly specific and rigorously validated in-house, RhoGDI Antibody (CST #2564) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RhoGDI Antibody detects endogenous levels of total RhoGDI protein. Species Reactivity: Human, Mouse, Rat, Bovine Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human RhoGDI. Antibodies are purified by protein A and peptide affinity chromatography. Background Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI). RhoGDI affects Rho activity by inhibiting nucleotide exchange and membrane association, regulating activity and localization (Reviewed in 1, 2). The inhibitory and shuttling functions of RhoGDI have been uncoupled using mutant forms of RhoGDI (3). Phosphorylation of GDIs and/or GTPases can modulate their affinity for each other and, therefore, GTPase mediated signaling. PAK1 phosphorylation of RhoGDI at serines 101 and 174 causes release and activation of Rac1, but not RhoA (4). Alternate Names ARHGDIA; epididymis secretory sperm binding protein Li 47e; GDIA1; GDIR1; GDP-dissociation inhibitor, aplysia RAS-related 1; HEL-S-47e; MGC117248; NPHS8; Rho GDI 1; Rho GDP dissociation inhibitor (GDI) alpha; Rho GDP dissociation inhibitor alpha; Rho GDP-dissociation inhibitor 1; Rho-GDI alpha; RHOGDI; RhoGDI alpha; RHOGDI-1 Specification REACTIVITY: H M R B SENSITIVITY: Endogenous MW (kDa): 26 SOURCE: Rabbit