CST, 25657S, TAP2 (E8G5I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TAP2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TAP2 (E8G5I) Rabbit Monoclonal Antibody (CST #25657) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TAP2 (E8G5I) Rabbit Monoclonal Antibody recognizes endogenous levels of total TAP2 protein. This antibody does not cross-react with TAP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val485 of human TAP2 protein. Background CD8 cytotoxic T cells recognize peptides presented by MHC class I molecules on the surface of infected cells and tumor cells. The transporters associated with antigen processing 1 and 2 (TAP1 and TAP2) form the TAP complex, which resides on the ER membrane and transports peptides from the cytoplasm into the ER for loading onto MHC class I molecules (1-8). In addition, TAP localized to endosomal membranes is important for cross-presentation by dendritic cells (9,10). IFN-γ produced by T cells and NK cells in response to infection causes upregulation of TAP1 and TAP2, resulting in increased antigen presentation to T cells (11). Some viral proteins inhibit TAP function or downregulate TAP expression, resulting in viral immune evasion (12,13). In addition, investigators have observed reduced TAP expression in a variety of tumor types, and it is thought to be one mechanism for tumor immune evasion (14). Alternate Names ABC transporter, MHC 2; ABC-transporter; ABC18; ABCB3; Antigen peptide transporter 2; APT2; ATP-binding cassette sub-family B member 3; ATP-binding cassette, sub-family B (MDR/TAP), member 3; D6S217E; peptide supply factor 2; peptide transporter involved in antigen processing 2; peptide transporter PSF2; peptide transporter TAP2; PSF-2; PSF2; really interesting new gene 11 protein; RING11; TAP2; transporter 2, ABC (ATP binding cassette); transporter 2, ATP binding cassette subfamily B member; transporter 2, ATP-binding cassette, sub-family B; transporter 2, ATP-binding cassette, sub-family B (MDR/TAP); Y1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 72 Source/Isotype: Rabbit IgG