CST, 25719S, NLRC4 (F7S2M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NLRC4 mouse. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NLRC4 (F7S2M) Rabbit Monoclonal Antibody (CST #25719) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NLRC4 (F7S2M) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRC4 protein. This antibody detects 28, 38, and 48 kDa proteins of unknown identity in some cell lines. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse NLRC4 protein. Background The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NACHT domain and carboxy-terminal leucine-rich repeats (LRR) involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7). NLRC4, also known as IPAF, can form inflammasomes in response to bacterial flagellin as well as components of the bacterial conserved type II secretion system (TTSS) (8-12). Ligand detection and ligand-dependent NLRC4 oligomerization and inflammasome activation require the NAIP family of proteins (13,14). In mice, NAIP5 and NAIP6 associate with flagellin, while NAIP2 interacts with TTSS rod proteins (13,14). In humans, NAIP recognizes the TTSS needle protein Cprl (14). In addition, NLRC4 is phosphorylated by PKCδ in response to bacterial infection, and this phosphorylation is required for inflammasome assembly and caspase-1 activation (15). Alternate Names 9530011P19Rik; Card; Card12; caspase recruitment domain family, member 12; Caspase recruitment domain-containing protein 12; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; Ice protease-activating factor; IPAF; NLR family CARD domain-containing protein 4; NLR family, CARD domain containing 4; Nlrc4 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG