CST, 25734S, hnRNP A2/B1 Antibody

Polyclonal Antibody for studying hnRNP A2/B1. Validated for Western Blotting. Highly specific and rigorously validated in-house, hnRNP A2/B1 Antibody (CST #25734) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity hnRNP A2/B1 Antibody detects endogenous levels of total hnRNP A2/B1 protein. This antibody does not cross-react with other hnRNP proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr41 of human hnRNP A2/B1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) is a member of the hnRNP A/B family of related RNA-binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). Alternative splicing produces transcripts that encode two homologous hnRNP proteins, hnRNPA2 and hnRNPB1, from a single gene sequence (2). Studies demonstrate hnRNP A2/B1 splicing repression across multiple targets (3,4) and that both proteins can bind and protect telomere repeat sequences from DNase digestion (5,6). Altered expression of hnRNP B1 is seen in several forms of cancer, including squamous cell carcinoma, adenocarcinoma, and various forms of lung cancer (7). Overexpression of hnRNP B1 may be associated with inhibition of DNA-PK activity and impaired DNA repair during early stages of cancer development (8). Autoantigens to hnRNP A2/B1 (termed RA33) are associated with rheumatoid arthritis, systemic lupus erythematosus, and mixed connective tissue disease (9-11). Alternate Names DKFZp779B0244; epididymis secretory sperm binding protein; FLJ22720; heterogeneous nuclear ribonucleoprotein A2/B1; heterogeneous nuclear ribonucleoprotein B1; Heterogeneous nuclear ribonucleoproteins A2/B1; hnRNP A2 / hnRNP B1; hnRNP A2/B1; HNRNPA2; HNRNPA2B1; HNRNPA2B1/MYC fusion; HNRNPB1; HNRPA2; HNRPA2B1; HNRPB1; IBMPFD2; nuclear ribonucleoprotein particle A2 protein; RNPA2; ROA2; SNRPB1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 34-36 SOURCE: Rabbit