CST, 2573S, Heregulin Antibody

Polyclonal Antibody for studying NRG1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Heregulin Antibody (CST #2573) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Heregulin Antibody detects endogenous levels of total heregulin proteins. This antibody does not cross-react with other family members. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His494 of human heregulin. Antibodies are purified by protein A and peptide affinity chromatography. Background Heregulin (HRG), also called neuregulin (NRG1), neu differentiation factor (NDF) or glial growth factor-2 (GGF-2), is a soluble growth factor synthesized as a transmembrane precursor molecule. Metalloproteinases and other proteases catalyze the cleavage of its extracellular domain which is then released and functions as a ligand for ErbB3 and ErbB4 receptor tyrosine kinase. The signaling pathways of HRG-ErbB3/ErbB4 are involved in regulation of cell proliferation, differentiation, invasion, and survival of both normal and malignant tissues (1,2). Abnormality of HRG-ErbB signaling leads to development of a variety of human diseases. HRG family has four isoforms including HRG-1, -2, -3 and -4, which are derived from alternative exon splicing. Moreover, they showed various tissue expression and biological activities (3). Alternate Names Acetylcholine receptor-inducing activity; ARIA; Breast cancer cell differentiation factor p45; GGF; GGF2; Glial growth factor; glial growth factor 2; Heregulin; heregulin, alpha (45kD, ERBB2 p185-activator); HGL; HRG; HRG1; HRGA; MST131; MSTP131; NDF; Neu differentiation factor; neuregulin 1; Neuregulin-1; NRG1; NRG1-IT2; Pro-neuregulin-1, membrane-bound isoform; Pro-NRG1; sensory and motor neuron derived factor; Sensory and motor neuron-derived factor; SMDF Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 105 SOURCE: Rabbit