CST, 25776T, TCL1 (F9R1X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TCL1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, TCL1 (F9R1X) Rabbit Monoclonal Antibody (CST #25776) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity TCL1 (F9R1X) Rabbit Monoclonal Antibody recognizes endogenous levels of total TCL1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TCL1 protein. Background T cell leukemia 1 (TCL1), MTCP1, and TCL1b belong to the TCL1 proto-oncogene family, and their products are involved in Akt activation during embryonic development and several types of T-cell leukemias and B-cell lymphomas (1-3). The Akt association domain of TCL1 binds with the PH domain of Akt. The formation of an oligomeric TCL-Akt complex is required for TCL1 coactivator function and results in phosphorylation and activation of Akt. Furthermore, functional analysis indicates that the interaction between TCL1 and Akt promotes translocation of Akt to the nucleus (4-6). These findings are supported by the crystal structure of TCL1, which suggests that TCL1 may participate in molecular transport (7). Alternate Names Oncogene TCL-1; Oncogene TCL1; Protein p14 TCL1; T cell leukemia/lymphoma 1A; T-cell leukemia/lymphoma 1A; T-cell leukemia/lymphoma protein 1A; T-cell lymphoma-1; T-cell lymphoma-1A; TCL1; TCL1 family AKT coactivator A; TCL1A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG