CST, 2578S, Histone H2A Antibody II

Polyclonal Antibody for studying H2A.1. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Histone H2A Antibody II (CST #2578) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity Histone H2A Antibody II detects endogenous levels of total histone H2A protein. This antibody also detects histone H2A.X and histone H2A.Z. Species Reactivity: Human, Mouse, Rat, Monkey, Zebrafish Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile79 of human histone H2A protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). Alternate Names FLJ92027; H2A clustered histone 13; H2A histone family, member C; H2A.1; H2A/c; H2A1; H2AC11; H2AC13; H2AC15; H2AC16; H2AC17; H2AFC; H2AFD; H2AFI; H2AFN; H2AFP; HIST1H2AG; HIST1H2AI; HIST1H2AK; HIST1H2AL; HIST1H2AM; histone 1, H2ai; histone cluster 1 H2A family member i; histone cluster 1, H2ai; Histone H2A type 1; Histone H2A/p; Histone H2A/ptl Specification REACTIVITY: H M R Mk Z SENSITIVITY: Endogenous MW (kDa): 14 SOURCE: Rabbit