CST, 2581S, Phospho-PP1 alpha (Thr320) Antibody

Polyclonal Antibody for studying PP1a (Thr320) phosphate. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Phospho-PP1 alpha (Thr320) Antibody (CST #2581) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-PP1 alpha (Thr320) Antibody detects PP1α only when phosphorylated at Thr320. This antibody may cross-react with the phosphorylated δ or γ isoforms of PP1. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr320 of human PP1α. Antibodies are purified by protein A and peptide affinity chromatography. Background Type 1 protein phosphatase (PP1), a serine/threonine phosphatase, is highly conserved in eukaryotic cells. Four isoforms of PP1 have been characterized: PP1α, PP1δ, PP1γ1 and PP1γ2 (1). Involvement in cell cycle regulation is one of the biological functions of PP1 (1). It has been illustrated that PP1 dephosphorylates Rb and cdc25 during mitosis (2,3). A cell cycle-dependent phosphorylation at Thr320 of PP1α by cdc2 kinase inhibits PP1α activity (4). Alternate Names MGC15877; MGC1674; PP-1A; PP1A; PP1alpha; PPP1A; PPP1CA; protein phosphatase 1 catalytic subunit alpha; protein phosphatase 1, catalytic subunit, alpha isoform; protein phosphatase 1, catalytic subunit, alpha isozyme; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; testicular tissue protein Li 155 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 38 SOURCE: Rabbit