CST, 25823S, SPAR (D9P9T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying lnc-HRCT1-1 / SPAR. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SPAR (D9P9T) Rabbit Monoclonal Antibody (CST #25823) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SPAR (D9P9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPAR protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human SPAR protein. Background Small regulatory Polypeptide of Amino Acid Response (SPAR) is a polypeptide, encoded by the lncRNA LINC00961 gene and is localized in late endosomal and lysosomal membranes. SPAR binds to the lysosomal v-ATPase complex in addition to ragulator, RagA and RagC. Upon amino acid stimulation, this supercomplex activates mTORC1 signaling. However, this process is inhibited by the binding of SPAR to the v-ATPase complex, as its formation prevents mTORC1 recruitment to lysosomes. SPAR downregulation and inactivation is an important focus for promoting muscle regeneration following muscle injury. This downregulation and inactivation promotes mTORC1 activation and results in myofiber maturation. In addition to muscle regeneration, SPAR downregulation and inactivation also promotes stem cell proliferation and differentiation. Alternate Names LINC00961; LOC158376; long intergenic non-protein coding RNA 961; Small regulatory polypeptide of amino acid response; small regulatory polypeptide of amino acids response; SPAAR; SPAR Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 13 Source/Isotype: Rabbit IgG