CST, 2585S, GLI2 (R770) Antibody

Polyclonal Antibody for studying GLI2. Validated for Western Blotting. Highly specific and rigorously validated in-house, GLI2 (R770) Antibody (CST #2585) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GLI2 (R770) Antibody detects transfected levels of human GLI2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg770 of human GLI2. Antibodies are purified by peptide affinity chromatography. Background GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Krüppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2, and GLI3 (3). These GLI proteins are similar to the homolog Cubitus interruptus (Ci) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development, and research studies have shown that this pathway is aberrantly activated in many types of cancers (4,5). GLI2 contains both transcription repression and activation domains (6) and several isoforms of GLI2 have been reported that may have different activities (7-9). Overexpression of GLI2 in skin causes basal cell carcinoma in mice (10), while loss-of-function of GLI2 is associated with pituitary anomalies (11). Alternate Names CJS; GLI family zinc finger 2; GLI family zinc finger protein 2; GLI-Kruppel family member GLI2; GLI2; glioma-associated oncogene family zinc finger 2; HPE9; oncogene GLI2; PHS2; Tax helper protein; tax helper protein 1; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP; THP1; THP2; Zinc finger protein GLI2 Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 220 SOURCE: Rabbit