CST, 2586T, PCNA (PC10) Mouse Monoclonal Antibody

Monoclonal Antibody for studying PCNA. Validated for WB,IP,IHC,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, PCNA (PC10) Mouse Monoclonal Antibody (CST #2586) is ready to ship. Product Usage Information Western Blotting: 1:2000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:8000 - 1:32000 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85118 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity PCNA (PC10) Mouse Monoclonal Antibody detects endogenous levels of PCNA protein. Species Reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T. Background Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation. Alternate Names ATLD2; Cyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen Specification REACTIVITY: H M R Mk B Pg SENSITIVITY: Endogenous MW (kDa): 36 Source/Isotype: Mouse IgG2a