CST, 25880SF, BAF (E4O8R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying BAF. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, BAF (E4O8R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #25880) is ready to ship. Product Usage Information This product is the carrier free version of product #98783. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 98783 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity BAF (E4O8R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total BAF protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala12 of human BAF protein. Background Barrier-to-autointegration factor (BAF or BANF1) plays a major role in the lifecycle of retroviruses, where it inhibits the reverse transcribed viral genome from integrating into itself (1-3). BAF exists as a dimer in solution, and serves as a bridge for double-stranded DNA in an ordered nucleoprotein complex (4). BAF interacts with LEM-domains of lamin binding proteins emerin and LAP2, and serves to help reform the nuclear envelope during telophase (5-7). The binding of BAF to its various partners is inhibited by Ser4 phosphorylation, which is deposited by vaccinia-related kinases (VRKs) and occurs during interphase and mitosis (8,9). Certain viral genomes encode kinases extremely similar to VRK to prevent BAF from binding to their DNA, impeding viral replication (10,11). BAF has been implicated in various tumor types and is associated with poor prognosis (12-14). Alternate Names BAF; BAF nuclear assembly factor 1; BANF1; barrier to autointegration factor 1; Barrier-to-autointegration factor; Barrier-to-autointegration factor, N-terminally processed; BCRG1; BCRP1; Breakpoint cluster region protein 1; D14S1460; MGC111161; NGPS Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 10,11 Source/Isotype: Rabbit IgG