CST, 2590S, SirT6 Antibody

Polyclonal Antibody for studying SirT6. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SirT6 Antibody (CST #2590) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SirT6 Antibody detects endogenous levels of total SirT6 protein. This antibody does not cross-react with other sirtuin proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the human SirT6 protein. Antibodies are purified by peptide affinity chromatography. Background The Silent Information Regulator (Sir2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of this family is Sir2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT6, a mammalian homolog of Sir2, is a nuclear, chromatin-associated protein that promotes the normal maintenance of genome integrity mediated by the base excision repair (BER) pathway (2-4). The BER pathway repairs single-stranded DNA lesions that arise spontaneously from endogenous alkylation, oxidation, and deamination events. SirT6 deficient mice show increased sensitivity to DNA-damaging agents, including the alkylating agents MMS and H O (2). In addition, these mice show genome instability with increased frequency of fragmented chromosomes, detached centromeres, and gaps (2). SirT6 may regulate the BER pathway by deacetylating DNA Polβ or other core components of the pathway (2). Alternate Names NAD-dependent deacetylase sirtuin-6; NAD-dependent protein deacetylase sirtuin-6; NAD-dependent protein deacylase sirtuin-6; Regulatory protein SIR2 homolog 6; SIR2-like protein 6; sir2-related protein type 6; SIR2L6; SIR6; SIRT6; sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae); sirtuin 6; sirtuin type 6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 SOURCE: Rabbit