CST, 25940T, SREBP-2 (F1X2D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SREBP-2. Validated for Western Blotting,Simple Western™,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SREBP-2 (F1X2D) Rabbit Monoclonal Antibody (CST #25940) is ready to ship. Product Usage Information For optimal ChIP results, use 2.5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Chromatin IP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity SREBP-2 (F1X2D) Rabbit Monoclonal Antibody recognizes endogenous levels of total SREBP-2 protein. This antibody does not cross-react with SREBP-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly40 of human SREBP-2 protein. Background Sterol regulatory element-binding proteins (SREBPs) are basic-helix-loop-helix-leucine zipper (bHLH-Zip) transcription factors. Inactive precursor forms of SREBPs are bound to endoplasmic reticulum (ER) membranes. When cells are starved for sterols, SREBPs move from the ER to the Golgi apparatus with the help of SREBP cleavage activating protein (SCAP). In the Golgi apparatus, precursor SREBPs are then sequentially cleaved by two proteases, site-1 protease (S1P) and site-2 protease (S2P). The released N-terminal domain that contains the bHLH-Zip region enters the nucleus and binds to sterol response elements (SREs) in the promoters of a variety of genes responsible for the synthesis of cholesterol, fatty acids, and other lipids, activating their expressions (1,2). Studies show that, in the tumor microenvironment, sterol metabolites oxysterols change the activities of SREBP-2 and LXR pathways and, therefore, cause T cell cholesterol deficiency, leading to T cell exhaustion and dysfunction (3). In addition, cholesin, a hormone secreted from the intestine as a reaction to cholesterol absorption, inhibits SREBP-2-mediated cholesterol production in the liver (4). Alternate Names BHLHD2; Class D basic helix-loop-helix protein 2; Processed sterol regulatory element-binding protein 2; SRBP2; SREBF2; SREBP-2; SREBP2; sterol regulatory element binding transcription factor 2; Sterol regulatory element-binding protein 2; Sterol regulatory element-binding transcription factor 2; Transcription factor SREBF2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-65, 124 Source/Isotype: Rabbit IgG