CST, 25959T, Aspartoacylase (F8X9Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ASPA. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Aspartoacylase (F8X9Z) Rabbit Monoclonal Antibody (CST #25959) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Aspartoacylase (F8X9Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total aspartoacylase protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro102 of human aspartoacylase protein. Background Aspartoacylase (ASPA), also known as aminoacylase-2 (ACY-2), is found primarily in oligodendrocytes and is the only enzyme in the brain that hydrolyzes N-acetyl-l-aspartate (NAA) (1,2). NAA is synthesized from l-aspartate and acetyl-CoA in the neuronal mitochondria of brain gray matter and is one of the most abundant amino acid derivatives found in the vertebrate brain, second only to glutamate (2). NAA levels in the brain must be tightly regulated, with abnormally low levels detected in multiple sclerosis and Alzheimer's disease, and abnormally high levels measured in Canavan disease (3-5). Canavan disease is a fatal neurodegenerative disorder and is the only identified genetic disorder caused by a defect in the processing of a metabolite produced exclusively in the brain (5). Alternate Names ACY-2; ACY2; aminoacylase 2; Aminoacylase-2; ASP; ASPA; Aspartoacylase; aspartoacylase (Canavan disease) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG