CST, 26013T, CD89/FCAR (F1T9J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FCAR. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, CD89/FCAR (F1T9J) Rabbit Monoclonal Antibody (CST #26013) is ready to ship. Product Usage Information Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85114 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity CD89/FCAR (F1T9J) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD89/FCAR protein. Non-specific staining was observed in pancreatic acinar cells and apical membrane of colon epithelium by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD89/FCAR protein. Background Immunoglobulin alpha Fc (IgA Fc) receptor (FcαRI), also known as CD89/FCAR, is a member of the Fc receptor superfamily and is expressed as a transmembrane glycoprotein on the surface of immune cells of the myeloid lineage (1-3). Research studies have demonstrated that CD89/FCAR can bind to polymeric IgA-containing immune complexes, which mediates receptor activation, internalization, and signaling (4). Productive signaling by CD89/FCAR requires association with the FcR gamma-chain and subsequent engagement of the tyrosine kinases, Fyn and Syk, to drive activation of pro-inflammatory signaling cascades (1,5). Alternate Names CD89; CTB-61M7.2; Fc alpha receptor; Fc fragment of IgA receptor; Fc fragment of IgA, receptor for; FcalphaRI; FCAR; FCAR variant 14; IgA Fc receptor; Immunoglobulin alpha Fc receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60-80 Source/Isotype: Rabbit IgG