CST, 26043T, B7-H3 (F9C1J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD276 mouse. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, B7-H3 (F9C1J) Rabbit Monoclonal Antibody (CST #26043) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity B7-H3 (F9C1J) Rabbit mAb recognizes endogenous levels of total B7-H3 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse B7-H3 protein. Background B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 protein contains two extracellular Ig-like V-type domains and two IgG-like C2-type domains, a transmembrane domain, and a short intracellular domain (1,2). Early research examining the biological process of B7-H3 suggested that B7-H3 is a positive regulator of T cell response (1). Subsequent research studies indicated that B7-H3 is a negative regulator of T cell response, and that the protein inhibits T cell proliferation (2,3). One possibility is that B7-H3 interacts with two distinct sets of receptors, resulting in seemingly opposite biological outcomes (2). B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate (1,4,5). Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature (6-8). Alternate Names 6030411F23Rik; AU016588; B7 homolog 3; B7-H3; B7h3; B7RP; B7RP-2; Cd276; CD276 antigen; Costimulatory molecule Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 (human), 50-55 (rodent) Source/Isotype: Rabbit IgG