CST, 2611S, Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody

Polyclonal Antibody for studying PKN1 (Thr774) phosphate/PKN2 (Thr816) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody (CST #2611) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-PRK1 (Thr774)/PRK2 (Thr816) Antibody detects endogenous levels of PRK1 and PRK2 only when phosphorylated at Thr774 or Thr816, respectively. This antibody also detects PKC zeta and lambda when phosphorylated at Thr 410 and 403, respectively. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr774 of human PRK1. Antibodies are purified by protein A and peptide affinity chromatography. Background The protein kinase C-related kinases (PRKs) are a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family (1-3). They are effectors of Rho family GTPases (4-6) and are activated by fatty acids and phospholipids (7,8). Activation and involves the activation loop phosphorylation of PRK1 (Thr774)/PRK2 (Thr816) by PDK1 (9,10). Alternate Names cardiolipin-activated protein kinase Pak2; DBK; MGC150606; MGC46204; MGC71074; PAK-1; Pak-2; PAK1; PAK2; PKN; PKN gamma; PKN-ALPHA; PKN1; PKN2; PRK1; PRK2; PRKCL1; PRKCL2; PRO2042; Protease-activated kinase 1; Protein kinase C-like 1; Protein kinase C-like 2; Protein kinase C-like PKN; protein kinase C-related kinase 1; protein kinase N1; protein kinase N2; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Protein-kinase C-related kinase 2; serine-threonine kinase N; Serine-threonine protein kinase N; serine/threonine protein kinase N; Serine/threonine-protein kinase N1; Serine/threonine-protein kinase N2; STK7 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 120 Phospho-PRK1. 140 Phospho-PRK2. SOURCE: Rabbit