CST, 26182S, RHBDF2 (E7U2K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RHBDL6. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RHBDF2 (E7U2K) Rabbit Monoclonal Antibody (CST #26182) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RHBDF2 (E7U2K) Rabbit Monoclonal Antibody recognizes endogenous levels of total RHBDF2 protein. Bands of unknown identity are detected at 50 kDa and 160 kDa. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys36 of human RHBDF2 protein. Background Inactive rhomboid factor 2 (RHBDF2), also known as RHBDL6 or iRhom2, is a proteolytically inactive member of the transmembrane family of rhomboid serine protease family (1). Inactive rhomboid family members have been implicated in several physiological and pathophysiological conditions, including wound healing, cancer, neurodegenerative diseases, and inflammatory disorders (reviewed in 2). RHBDF2 facilitates endoplasmic reticulum trafficking of the metalloprotease ADAM17 (also known as TACE) to the membrane, thereby promoting EGF family growth factor and cytokine shedding and signaling (1). Loss of RHBDF2 results in significantly reduced secretion of ligand-mediated secretion of these factors. Inherited dominant mutations in the gene are the genetic basis for the cancer-susceptibility syndrome tylosis with esophageal cancer (TOC) (3). Elevated expression of RHBDF2 in oral squamous carcinoma is associated with tumor progression and poor patient prognosis (4). The E3 ubiquitin ligase TRIM31 directly targets RHBDF2 and facilitates its proteasomal degradation (5). Hepatocyte specific ablation of TRIM31 facilitates phenotypes of non-alcoholic fatty liver disease (5). Alternate Names FLJ22341; Inactive rhomboid protein 2; IRHOM2; RHBDF2; RHBDL5; RHBDL6; RHDF2; Rhomboid 5 homolog 2; rhomboid 5 homolog 2 (Drosophila); Rhomboid family member 2; rhomboid veinlet-like 5; Rhomboid veinlet-like protein 5; Rhomboid veinlet-like protein 6; rhomboid-like protein 6; rhomboid, veinlet-like 6; TEC; TOC; TOCG Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 97 Source/Isotype: Rabbit IgG