CST, 26233T, CD34 (E2J1K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD34 mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD34 (E2J1K) Rabbit Monoclonal Antibody (CST #26233) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD34 (E2J1K) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD34 protein. Nuclear background may be observed in cultured cell lines by immunofluorescence. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with mouse CD34 recombinant protein. Background CD34 is a type I transmembrane glycophosphoprotein expressed by hematopoietic stem/progenitor cells, vascular endothelium and some fibroblasts (1). CD34 expression has been the hallmark used to identify hematopoietic stem cells for many years. CD34+ hematopoietic stem cells expand and differentiate into all the lymphohematopoietic lineages upon cytokine or growth factor stimulation and lose CD34 expression upon differentiation. However, recent studies performed in various laboratories conflict with that convention (2). The extracellular domain of CD34 is homologous to CD43, a protein involved in cell-cell adhesion, and CD34 has been shown to function as a negative regulator of cell adhesion (3). CD34 associates with CrkL but not CrkII, is a substrate for PKC, and activation of PKC is coupled with surface expression of CD34 (1,4). Alternate Names AU040960; Cd34; CD34 antigen; cluster designation 34; Hematopoietic progenitor cell antigen CD34 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 80-120 Source/Isotype: Rabbit IgG