CST, 26254S, Posibeads™ Whitlow/218 Linker Positive Control Beads

Affinity and Purification Reagents for studying in the research area. Product Usage Information Posibeads™ can be used as an independent positive control sample or mixed with cells to function as an internal positive control. Recommended usage is 5 µL (around 100,000 beads) for use as an independent sample or 1 µL when mixed with cells. (If desired, perform a titration to determine the optimal ratio of beads to cells for your experiment.) 1. Prepare cells/tissue in tubes or wells as necessary for your experiment. If Posibeads™ will be used as an independent positive control, add an equivalent volume of buffer to empty tube(s) or well(s) as desired. NOTE: Plan to include at least one unstained control to establish background fluorescence in your channel(s) of interest. 2. Mix or vortex the Posibeads™ immediately prior to use to ensure the beads are evenly distributed in solution. 3. Add desired volume of Posibeads™ to the control tube(s) or well(s), or add directly into the cell samples for use as an internal control. 4. Proceed with immunostaining following established protocols. The same incubation and wash steps should be performed with samples of interest and the Posibead control samples. The same centrifugation conditions may be used for both Posibeads™ and your cells of interest. 5. Acquire samples on flow cytometer. In mixed samples, beads will be differentiated from cells by high side scatter and low forward scatter. Storage Store at 4 ºC. Supplied in 1X PBS (pH 7.4), 0.05% Tween 20, and 0.05% sodium azide.