CST, 2625S, GST-Tag (91G1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, GST-Tag (91G1) Rabbit Monoclonal Antibody (CST #2625) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GST-Tag (91G1) Rabbit Monoclonal Antibody detects transfected GST fusion proteins. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a GST fusion protein. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Rabbit IgG