CST, 2630S, Atg5 Antibody

Polyclonal Antibody for studying ATG5. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Atg5 Antibody (CST #2630) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Atg5 Antibody detects endogenous levels of total Atg5. The observed band represents the Atg12-Atg5 conjugated form, but the antibody likely reacts with free Atg5 as well. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy-terminus of Atg5. Antibodies were purified by peptide affinity chromatography. Background Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related ( ) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). Alternate Names APG5; APG5 (autophagy 5, S. cerevisiae)-like; APG5 autophagy 5-like; APG5-like; APG5L; apoptosis specific protein; Apoptosis-specific protein; ASP; ATG5; ATG5 autophagy related 5 homolog; ATG5 autophagy related 5 homolog (S. cerevisiae); ATG5 autophagy related 5-like; Autophagy protein 5; autophagy related 5; hAPG5; SCAR25 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 55 SOURCE: Rabbit