CST, 2637S, BTAF1 Antibody

Polyclonal Antibody for studying TAF172. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, BTAF1 Antibody (CST #2637) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BTAF1 Antibody recognizes endogenous levels of total BTAF1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala220 of human BTAF1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background B-TATA binding protein-associated factor 1 (BTAF1) is a human homolog of the yeast protein modifier of transcription 1 (MOT1) (1). BTAF1 negatively regulates transcription through interaction with TATA binding protein (TBP), an important component of the B-TFIID complex (2,3). BTAF1 is a member of the SNF2 family of ATPases that utilizes ATP to remove TBP from B-TFIID, thereby preventing formation of the transcription pre-initiation complex (PIC) (3-5). BTAF1 actively removes TBP from DNA, which prevents non-specific TBP-DNA interactions and promotes disassembly of inactive forms of the PIC. This activity is thought to be critical for maintaining a pool of free TBP that is necessary for the assembly of the PIC during transcription activation (6,7). Alternate Names ATP-dependent helicase BTAF1; B-TFIID TATA-box binding protein associated factor 1; B-TFIID transcription factor-associated 170 kDa subunit; BTAF1; BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae); KIAA0940; MGC138406; MOT1; Mot1 homolog; TAF-172; TAF(II)170; TAF172; TAFII170; TATA-binding protein-associated factor 172; TBP-associated factor 172 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 200 SOURCE: Rabbit